Dipl-Ing. Stefanie Schuldt

Stefanie Schuldt began studying Biochemical Engineering at the TU Dortmund in 2003 and finished her degree in 2009 with the diploma thesis "Selection of Sorbents using Purification Fingerprints within a Systematic Downstream Process Design".

Since 2009 to 2012 she worked as a PhD student at the laboratory of Plant and Process Design. The focus of her research was "Experimentally based systematic Downstream Process Design".

The performance of a process depends heavily on the decisions made during early stages of downstream process development. Especially for biotechnological products the downstream process often accounts for more than 50% of the total production costs. The aim of this thesis is to develop a method for systematic downstream process design in early stages of process synthesis. Therefore different unit operations, mass separating agents and operating conditions are investigated and compared. This experimental investigation of different process steps is carried out with high-throughput technologies. High-throughput screening (HTS) is a relatively new micro scale technique which allows to quickly prepare and analyze a multiplicity of samples. The application of HTS technologies for downstream process design is a comparatively new field of application. Because of this the development of suitable strategies for the application of this technique is one aspect of this thesis. The experimental approach will be combined with theoretical algorithmic methods. Based on key performance indicators the most promising process alternatives will be used to form an efficient downstream process.

The major objective is to develop a strategy for rapid and comprehensive downstream process development of biotechnological products.

The evaluation of these methods focuses on the isolation of biotechnological products from fermentation broth, for example the purification of secondary metabolites from the supernatant of basidiomycetes.

Paper

• S. Schuldt, G. Schembecker
  A Fully Automated Ad- and Desorption Method for Resin and Solvent Screening
  Chemical Engineering and Technology, 2013, Vol. 36 (7), p. 1157 -1164, doi.org/10.1002/ceat.201200725
   
• S. Schuldt, T. Winkelnkemper, G. Schembecker
  Entwicklung von Downstream-Prozessen
  in Chmiel, Hortst (Hrsg.), Bioprozesstechnik (3. Auflage) Springer Verlag, Heidelberg, (2011), S. 363-368
   
• T. Winkelnkemper, S. Schuldt, G. Schembecker,
  Systematic downstream process development for purification of baccatin III with key performance indicators
  Separation and Purification Technology, 2011, vol. 77 (3), p. 355-366, doi.org/10.1016/j.seppur.2011.01.004

Oral presentations

• S. Schuldt, G. Schembecker
  Automation techniques in systematic process development for purification of natural substances
  European Workshop on High-Throughput Developments and Applications, Wildbad Kreuth (2012)
   
• S. Schuldt, G. Schembecker
  Automation of ad- and desorption experiments: An alternative approach for downstream process synthesis
  16th International Conference on Biopartitioning and Purification (BPP), Puerto Vallarta, Mexico (2011)
   
• S. Schuldt, T. Winkelnkemper, G. Schembecker
  Key Performance Indicators for Downstream Process Development
  28. DECHEMA-Jahrestagung der Biotechnologen und ProcessNet-Jahrestagung, Deutschland (2010)
   
• T. Winkelnkemper, S. Schuldt, G. Schembecker
  Kennzahlengesteuerte systematische Downstream Prozessentwicklung
  GVC / Dechema Vortrags- und Diskussionstagung: Bioprozessorientiertes Anlagendesign, Nürnberg, Deutschland (2010)
   
• T. Winkelnkemper, S. Schuldt, G. Schembecker
  Systematic Downstream Process Design: Purification of Taxanes with Key Performance Indicators
  First Annual CLIB-Graduate Cluster Retreat, Wuppertal, Deutschland (2010)

Poster presentations

• S. Schuldt, G. Schembecker
  Automation Techniques in Systematic Process Development for Purification of Natural Substances
  ProcessNet Jahrestagung, Karlsruhe, Deutschland (2012)
   
• S. Schuldt, G. Schembecker
  Automation techniques in experimental based process development
  3rd Annual CLIB GC Retreat, Bergisch Gladbach (2012)
   
• S. Schuldt, G. Schembecker
  Automation of ad- and desorption experiments: An alternative approach for downstream process synthesis
  8th European Congress of Chemical Engineering, Berlin, Deutschland (2011)
   
• S. Schuldt, G. Schembecker
  Systematic Downstream Process Design: Robotic Systems
  First Annual CLIB-Graduate Cluster Retreat, Wuppertal, Deutschland (2010)
   
• S. Schuldt, G. Schembecker
  A novel Method to design Ad- and Desorption Processes for Purification of Active Pharmaceutical Ingredients (API) using a Robotic Platform
  Second Annual CLIB-Graduate Cluster Retreat, Bonn, Deutschland (2011)