Dr.-Ing. Christoph Schwienheer
Curriculum Vitae
Christoph Schwienheer studied biochemical engineering at the Technical University of Dortmund since 2005. He graduated in 2010 with a diploma thesis dealing with modeling and experimental research of hydrodynamics in a chamber of the hydrostatic centrifugal partition chromatography (CPC).
From 2009 til 2015 he worked as PhD student at the Laboratory of Plant and Process Design at the Technical University of Dortmund. His research was about centrifugal partition chromatography.
Field of Research
Centrifugal partition chromatography (CPC) is a preparative method mainly used for purification of natural products.
The separation principle is based on different partitioning of substances between two immiscible liquid phases. One phase is kept stationary in chambers using a centrifugal field while the mobile phase is pumped through this stationary phase. As for example comparable to liquid chromatography a time resolute separation between the substances occurs.
Using aqueous two-phase systems (ATPS) as solvent system it is possible to use the CPC in the downstream process of biotechnological products (e.g. proteins, antibodies). The special physical properties of ATPS offer a conservative application on the one hand, but special requirements for the usage in CPC on the other hand.
The aim of this project is to make the usage of ATPS in CPC possible. At first suitable operation conditions (e.g. CPC chambers geometry, volume flow, revolution) have to be explored. After this it is possible to clean biotechnological products using the modified CPC
Publications
Patent
- C. Schwienheer, S. Adelmann, G. Schembecker
Extraktor für eine Zentrifugalverteilungsextraktion und Verfahren zur Durchführung einer Zentrifugalextraktion
PCT/EP2012/068999, 26.09.2012
Dissertation
- C. Schwienheer
Advances in centrifugal purification techniques for separating ( bio-) chemical compounds
TU Dortmund, 2016, Verlag Dr. Hut, München ISBN 978-3-8439-2916-5
Paper
- C. Schwienheer, J. Krause, G. Schembecker, J. Merz
Modelling centrifugal partition chromatography separation behavior to characterize influencing hydrodynamic effects on separation efficiency
Journal of Chromatography A, 1492 ( 2017) 27–40, doi.org/10.1016/j.chroma.2017.02.055
- C. Schwienheer, A. Prinz, T. Zeiner, J. Merz
Separation of active laccases from Pleurotus sapidus culturesupernatant using aqueous two-phase systems in centrifugal partition chromatography
Journal of Chromatography B 1002 (2015) 1–7, doi.org/10.1016/j.jchromb.2015.07.050
- C. Schwienheer, J. Merz, G. Schembecker
Selection and use of poly ethylene glycol and phosphate based aqueous two-phase systems for the separation of proteins by Centrifugal Partition Chromatography
Journal of Liquid Chromatography & Related Technologies, 2015, doi.org/10.1080/10826076.2014.951765
- C. Schwienheer, J. Merz, G. Schembecker
Investigation, comparison and design of chambers used in Centrifugal Partition Chromatography on basis of flow pattern and separation experiments
Journal of Chromatography A ,1390 (2015) 39-49, doi.org/10.1016/j.chroma.2015.01.085
- S. Adelmann, C. Schwienheer, G. Schembecker
Multiphase flow modeling in Centrifugal Partition Chromatography
Journal of Chromatography A, Volume 1218, Issue 36, Page 6092-6101 (2011), doi.org/10.1016/j.chroma.2011.01.063
Oral presentations
- C. Schwienheer, J. Merz, G. Schembecker
Selection and use of aqueous two-phase systems for the separation of bio-active compounds with Centrifugal Partition Chromatography
8. Doktorandenseminar "`Präparative Chromatographie"', Delft, Netherlands (2014)
- C. Schwienheer, J. Merz, G. Schembecker
Evaluation of CPC separation efficiency for different types of chamber geometries on the basis of flow pattern and separation experiments
8th International Conference on CounterCurrent Chromatography (CCC 2014), London, United Kingdom (2014)
- C. Schwienheer, J. Merz, G. Schembecker
Selection and use of aqueous two-phase systems for the separation of bio-active compounds with Centrifugal Partition Chromatography
ProcessNet Jahresttagung der DECHEMA und VDI-GVC, Aachen, Germany (2014)
- C. Schwienheer, J. Merz, G. Schembecker
A new high performance continuous extractor based on Centrifugal Partition Chromatography
8. Doktorandenseminar "Präparative Chromatographie", Weggis, Switzerland (2013)
- C. Schwienheer, J. Merz, G. Schembecker
Continuous Counter Flow Centrifugal Partition Extraction as a new method for small scale extraction processes
ProcessNet "Jahrestreffen der Fachausschüsse Extraktion und Mehrphasenströmungen", Baden-Baden, Germany (2013)
- C. Schwienheer, J. Merz, G. Schembecker
Countercurrent Centrifugal Extraction: A high efficient extraction apparatus
ProcessNet "`Jahrestreffen der Fachgemeinschaft Fluiddynamik und Trenntechnik"', Würzburg, Germany (2013)
- C. Schwienheer, J. Merz, S. Adelmann, G. Schembecker
Selection and use of aqueous two phase systems in centrifugal partition chromatography
7th International Conference on CounterCurrent Chromatography (CCC 2012), Hangzhou, China (2012)
- C.Schwienheer, M. Fittkau, G.Schembecker, J.-C. Antonie, A. Liese
Investigation of enzyme-catalyzed reactions in Centrifugal Partition Chromatography as a reactive separation technique in biotechnological processes
Downstream Days 2012, Wetter, Germany (2012)
- C. Schwienheer, S. Adelmann, G. Schembecker
Simulation und experimentelle Untersuchung der Hydrodynamik in einem Zentrifugalchromatographen
OpenFOAM Workshop, Dortmund, Germany (2011)
- C. Schwienheer, S. Adelmann, G. Schembecker
Hydrodynamic analysis in Centrifugal Partition Chromatography using experimental visualization and multiphase flow modeling
7. Doktorandenseminar "Präparative Chromatographie", Wernigerode, Germany (2011)
- C. Schwienheer, S. Adelmann, G. Schembecker
Simulation und experimentelle Untersuchung der Hydrodynamik in einem Zentrifugalchromatographen
ProcessNet "Jahrestreffen der Fachausschüsse Extraktion, Fluidverfahrenstechnik, Mehrphasenströmungen und Phytoextrakte", Fulda, Germany (2011)
Poster presentations
- C. Schwienheer, J. Merz, G. Schembecker
Aqueous two-phase systems for the gentle separation of bio-active compounds using centrifugal partition chromatography
8th International Conference on Countercurrent Chromatography, London, UK (2014)
- C. Schwienheer, S. Adelmann, G. Schembecker
Selection and use of Aqueous Two Phase Systems in Centrifugal Partition Chromatography
Himmelfahrtstagung der DECHEMA: Effiziente Verfahren und Anlagen in der Bioverfahrenstechnik und Lebensmittelbiotechnologie, Bad-Wildungen, Germany (2013)
- C. Schwienheer, S. Adelmann, G. Schembecker
Countercurrent Centrifugal Extraction: A new and high efficient extraction apparatus based on Centrifugal Partition Chromatography
17th International Conference on Biopartitioning and Purification (BPP 2013), Newport (RI), USA (2013)
- C. Schwienheer, J. Merz, S. Adelmann, G. Schembecker
Selection and use of aqueous two phase systems in centrifugal partition chromatography
9th European Symposium on Biochemical Engineering Science, Istanbul, Turkey (2012)
- C. Schwienheer, S. Adelmann, G. Schembecker
Auswahl und Einsatz wässriger Zweiphasensysteme in der Centrifugal Partition Chromatography
ProcessNet Jahrestagung, Karlsruhe, Germany (2012)